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. 2012 Mar 13;3:738. doi: 10.1038/ncomms1744

Figure 6. Zolpidem and THIP reveal changes of α1-δ subunit expression in altered KCC2 expression.

Figure 6

(a) Summary of the average percent increase in the sIPSC decay kinetics in KCC2 and KCC2 shRNA transfected neurons by application of Zolpidem (at DIV6–7 control n=6; KCC2 over n=9 *P<0.05; ANOVA; at DIV12–13 control n=9; KCC2 shRNA n=10; **P<0.01; ANOVA). (b) Representative whole-cell responses in KCC2 and KCC2 shRNA transfected neurons to applications of THIP (30 μM) and GABA (1 mM). (c) Summary of the average current density ITHIP/IGABA in KCC2 transfected neurons (control, grey bar n=22; KCC2 over, black bar n=22; ***P<0.001 ANOVA). (d) Summary of the average current density ITHIP/IGABA in KCC2 shRNA transfected neurons (control, grey bar n=12; KCC2 shRNA, white bar n=12; ***P<0.001 ANOVA). Data is presented as mean±s.e.m.