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. 2012 Mar 13;3:738. doi: 10.1038/ncomms1744

Figure 8. Transfection of KCC2 shRNA at mature developmental stage affects the α3-α1 and δ subunit expression.

Figure 8

(a) Representative sIPSCs traces in control and KCC2 shRNA transfected neurons at DIV14. (b) Summary of sIPSC decay kinetics in control and KCC2 shRNA transfected neurons (control, grey bar n=18; KCC2 shRNA, white bar n=25; **P<0.01; unpaired t-test). (c) Summary of tonic currents in control and KCC2 shRNA transfected neurons (control, grey bar n=10; KCC2 shRNA, white bar n=24; **P<0.01; unpaired t-test). (d) Representative somatic immunostaining of α3, α1 and δ subunits in control and KCC2 shRNA transfected neurons. (e) Quantification of immunofluorescence signals for α3 and α1 subunits (α3 control, grey bar n=16; KCC2 shRNA, white bar n=23; ***P<0.001; unpaired t-test; α1 control, grey bar n=16; KCC2 shRNA, white bar n=12; *P<0.05; unpaired t-test). (f) Quantification of immunofluorescence signals for δ subunit (control, grey bar n=34; KCC2 shRNA, white bar n=16; ***P<0.001; unpaired t-test). Scale bar: 23 μm. Data is presented as mean±s.e.m.