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. 2012 Mar 6;3:713. doi: 10.1038/ncomms1726

Figure 4. Function of the upstream insertion in transgenic barley.

Figure 4

(a) Expression of HvAACT1 in the root tips (0–3 mm). Plasmids containing HvAACT1 ORF fused with Murasakimochi promoter (B1), Morex promoter (B2) or insertion-originated promoter (B3) were transformed into the barley cultivar Golden Promise (GP). (b) Al-induced secretion of citrate. (c) Al tolerance in transgenic barley lines. (d–f) Expression of HvAACT1 in the root tips (0–3 mm) (d), Al-induced secretion of citrate (e) and Al tolerance in transgenic barley lines (f) in a separate experiment using different independent lines. Data are shown as the means±s.d. (n=3) for a,b,d,e and (n=10) for c,f. Statistical comparison was performed by one-way ANOVA followed by the Tukey's multiple comparison test. All data were compared with GP. *P<0.05.