Figure 5. Complementation test for iron translocation in rice.
(a) Rescue of leaf chlorosis by the introduction of Morex HvAACT1. (b,c) SPAD value of the youngest expanded leaf of transgenic rice carrying Morex-type HvAACT1 promoter grown in a nutrient solution containing 0.2 (b) or 10 (c) μM FeSO4. Data are shown as means±s.d. (n=4–6). (d,e) Concentration of citrate (d) and iron (e) in the xylem sap of different rice lines. (f,g) Concentration of iron in the shoots (f) and roots (g). Data are shown as means±s.d. (n=3–5). Statistical comparison was performed by one-way ANOVA followed by Dunnett's multiple comparison test. *P<0.05. All data were compared with osfrdl1. (h) Rescue of iron precipitation in the roots by the introduction of Morex HvAACT1. Scale bars in a and h indicate 10 mm and 100 μm, respectively.