Table 1. Key properties of novel CFP variants.
| Protein | ɛ* (103 M−1 cm−1) | QY† |
Brightness |
Photobleaching∥ (%) |
Lifetime (ns) |
||
|---|---|---|---|---|---|---|---|
| In vitro‡ | In vivo§ | τϕ | τM | ||||
| mTurquoise2 | 30 (434) | 0.93±0.02 (474) | 110 | 118 | 3±1 | 3.8 | 4.0 |
| mTurquoise-146G | 25 | 0.61±0.01 | 63 | 59 | ND | 2.9 | 3.2 |
| mTurquoise-146S | 20 | 0.48±0.02 | 58 | 38 | ND | 2.4 | 2.8 |
| mTurquoise | 30¶ (434) | 0.84±0.02 (474) | 100 | 100 | 3±1 | 3.7 | 3.8 |
| mCerulean3 | 30 | 0.80±0.01 | 100 | 95 | 39±2 | 3.7 | 3.8 |
| SCFP3A | 30¶ | 0.56¶ | 67¶ | 67¶ | 7±1 | 2.9¶ | 3.3¶ |
| ECFP |
30¶ |
0.36¶ |
40¶ |
40¶ |
13±2 |
2.3¶ |
3.0¶ |
*Extinction coefficient with absorbance maximum (nm) between brackets.
†Quantum yield (average±standard deviation (s.d.)) with emission maximum (nm) between brackets.
‡Determined from photon-counting histogram analysis (relative to mTurquoise).
§In HeLa cells (relative to mTurquoise).
∥Photobleaching (average±s.d.) is determined by the reduction in fluorescence (1−F/F0) in living cells after 150 s illumination at 1.6 W cm−2 (ND=not determined).
¶Previously reported values10.