Figure 5.

Regulator of cell fusion is suppressed in OCLs depleted of Orai1. A) Infected RAW cells were cultured with or without RANKL for 2 d, and qRT-PCR analysis was performed to examine the expression of ATP6v0d2 induced by RANKL. Statistical significance was evaluated by an ANOVA. Data represent 3 independent experiments with similar results. B) Left panel: Western blot analysis of ATP6v0d2 from RAW cells cultured with or without RANKL for 2 d. Right panel: densitometric analyses of ATP6v0d2 protein levels were performed from 3 independent experiments. Data represent means ± se. C) qRT-PCR analysis was performed to examine the expression of DC-STAMP. *P < 0.05, **P < 0.01, ***P<0.001.