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. 2012 Apr;26(4):1640–1651. doi: 10.1096/fj.11-199216

Figure 3.

Figure 3.

Characterization of IL-17A release by macrophages. A) Flow cytometry analysis for intracellular cytokine staining for IL-17A as a function of the macrophage surface markers, CD11b and F4/80. B) IL-17A release from LPS-activated PEMs from C57BL/6J mice compared to cells from γδ T-cell-deficient mice. C) Release of IL-17A from PEMs from αβ T-cell-deficient mice after LPS activation. D) Release of IL-17A from Wt macrophages after stimulation with agonists for TLR2, TLR3, TLR4, TLR5, and TLR9 (all at 1 μg/ml). E) Release of IL-17A from LPS-activated PEMs from C57BL/6J mice and MyD88−/− mice. F) RT-PCR analysis of mRNA levels for IL-17A in LPS-stimulated PEMs from C57BL/6J mice and MyD88−/− mice. Control (Ctrl) denotes untreated PEMs in cell culture medium alone. LPS (1 μg/ml) and 12 h incubation for all experiments. Error bars represent means ± se. **P < 0.01, ***P < 0.001; Student's t test.