Figure 4.

Maresin 1 stimulates tissue regeneration. A) Anterior portions of brown planaria were surgically removed, and remaining planaria were exposed to vehicle (squares), RvE1 (100 nM; circles), or MaR1 (100 nM; triangles) for 7 d. B) Planaria were exposed to increasing concentrations of MaR1 (circles, 1 nM; triangles, 10 nM; diamonds, 100 nM), and regeneration was assessed for 7 d. C) Concentration dependence. D) Planaria 4 d after surgery with or without MaR1 were stained using fluorescently conjugated ECL to visualize secretory cells; see Materials and Methods. Inset (left panel): gross whole worm. Results are means ± se (n=5 planaria/group). *P < 0.05, **P < 0.01, ***P < 0.001 vs. vehicle alone.