Figure 5.

Estradiol regulates epithelial wound healing and LXA₄ formation. A) HCE monolayers were treated with estradiol (1 pM) for 24 h. Cells were harvested, and 5-LOX and 15-LOX mRNA expression was quantified by qPCR (n=6). B) Epithelial LXA₄ formation. HCE cells were treated with estradiol (1 pM) or pure medium for 24 h, cells were activated with calcium ionophore for 15 min, and medium was collected for LC/MS/MS quantification of endogenous LXA₄ formation (n=6). C) In vitro epithelial wound healing. Confluent cells were incubated with or without LXA₄ (1 nM) in the presence or absence of estradiol (1 pM) for 24 h prior to scratch wounding. Wound healing was assessed at 8 h postinjury and is expressed as percentage area healed (n=5). D) Representative image of epithelium at 8 h after scratch injury. Lines indicate initial wound area. *P < 0.05 vs. control; ^#P < 0.05 vs. estradiol treatment.