Figure 3.

Nonpolar and polar blueberry fractions are not cytotoxic. Serum-free cultures of SH-SY5Y cells were supplemented with 5, 30, and 75 μg/mL of nonpolar blueberry fraction (NP_BB) or polar blueberry fraction (PO_BB) for 48 h. Cell viability was measured using an MTT assay, and all values were normalized to control (CON). Neither NP_BB nor PO_BB blueberry fractions compromised cell viability compared to our positive control (H₂O₂, 48 h). Notably, all NP_BB or PO_BB concentrations tested potently negated ROS formation in SH-SY5Y cells exposed to TNFα (200 ng/mL) or PMA (400 ng/mL). Data represent the mean of eight independent experiments ± standard deviations, and statistical significance was determined at *P < 0.05  (ANOVA and Tukey's post hoc analysis).