Figure 4.

PPARα activation could not improve Foxp3 promoter activity. (a) MEF cells were transfected with Foxp3 promoter-luciferase reporter plasmid along with β-galactosidase plasmid, then stimulated with 20 μM fenofibrate or further transfected with PPARα plasmid. All groups received equal amount of solute and plasmid adjusted with ethanol and GFP plasmid. (b) Foxp3 promoter-luciferase reporter plasmid and β-galactosidase plasmid were transfected into MEF cells and then the cells were stimulated with 50 ng/mL PMA and 1 μg/mL ionomycin. PPRE-luciferase reporter plasmid and β-galactosidase plasmid were transfected to MEF cells with PPARα expression plasmid or equal amount of GFP control plasmid (c) or to HEK 293 cells that were immediately stimulated with 20 μM fenofibrate, 50 μM gemfibrozil or 100 μM WY14643 (d). 24 hours later, luciferase activity relative to β-galactosidase activity was analyzed. n = 5, *P < 0.05.