Table 2.
Experimental design of the formal study.
| Group | Time | |||||
|---|---|---|---|---|---|---|
| −0.5 h | 0 h | 1 h | 3 h | 6 h | 12 h | |
| CON | Vehicle (10% DMSO) | Saline | Sacrifice (n = 16) | Sacrifice (n = 16) | Sacrifice (n = 16) | Sacrifice (n = 16) |
| TC | Vehicle (10% DMSO) | Taurocholate | Sacrifice (n = 16) | Sacrifice (n = 16) | Sacrifice (n = 16) | Sacrifice (n = 16) |
| ZP | Zerumbone 10 mg/kg | Taurocholate | Sacrifice (n = 16) | |||
| Z | Zerumbone 10 mg/kg | Saline | Sacrifice (n = 16) | |||
In the TC group, rats received the vehicle (10% DMSO, 2 mL/kg) administered via femoral vein half an hour prior to retrograde infusion of a freshly prepared 5% sodium taurocholate solution (1 mg/kg). In the CON group, rats received the vehicle administered via femoral vein. After half an hour, the rats were retrograde infused isotonic saline solution (1 mL/kg). In the ZP group, rats received 10 mg/kg zerumbone dissolved in vehicle (10% DMSO v/v) administered via femoral vein half an hour prior to infusion 5% sodium taurocholate solution (1 mg/kg). In the Z group, rats received the same as ZP group except infusion isotonic saline solution instead of taurocholate.