Table 1.

Primers and PCR amplification conditions

Name	Type*	Alleles†	Species	Primer pairs		PCR‡
				Markers
BMS2508	ms	3	Cattle	AGGTTGACTTCTGTGTCTTTTC	GTTTCTTAGGGGAGTGTTGATT	56/2.5
ETL1	snp	2	Sheep	CATCAATGGGCCTGTGAAAT	CAAACAATCACCAACTAATCC	54/3
471U	ms	3	Sheep	CTACAATAAATAATGAGGTGAAA	TGAGTAGAGACAAAGCTATAAA	52/3
UNC5C (GC101)	ms	7	Sheep	ATCCTCACCCTTCAAACAG	CTGGGGAGTTTTCTCTGAC	56/1.5
UNC5C (GC102)	snp	3	Sheep	TTCCCTCTTGCTGTATCTTCT	AAACACTGGGTGACTGACTC	58/1.5
300R	snp	3	Sheep	CCAAACATCAGCAAACCATAA	CTGTGACCATCTCTCTGTG	56/3
320R	snp	4	Sheep	TCACCTTGAATCATTTCTTTAG	GTGGTGTTTGGAACTTTGTAA	55/1.5
300U	ms	12	Sheep	GGGGGTTCCTTGTAGGTTTGTG	GGAAGTGCAGAGAGTCCCATAC	66/3
Bulge5	ms	6	Cattle	CACAAACATTCGGACATGACC	TGAGGAGGGCACATCCATTG	54/3
LSCV043	ms	6	Goat	CCAGAATATAGAGTTTTGTCAAG	GCCTGATTTGTATTTGTATGAG	53/3
				BMPR-IB analysis
OB1	snp	4	Sheep	CAGAACGGAATGAATGTAATAA	AGAACAGGGTAAGGAAGTAAA	55/3
OB2	snp	2	Sheep	AGATTGGAAAAGGTCGCTATG	ACCCTGAACATCGCTAATACA	56/1.5
B3	cDNA		Human	TGACAAGAGAGGAAACAAAAAG	AGGACCAAGAGCAAACTACA	55/2
B4	cDNA		Human/sheep	GACACTCCCATTCCTCATCA	GCAGTTTCTCCCGCCTACAG	55/2

^*ms, microsatellite; snp, single nucleotide polymorphism; cDNA, primers for cDNA amplification. 

^† Number of alleles in the sheep backcross population. 

^‡ Annealing temperature (°C) and magnesium concentration (mM).