Figure 4.

C2C12 cells were seeded onto chamber slides and allowed to differentiate from myoblasts to myotubes as described in the Materials and Methods section. Myotubes were then transfected at each concentration of siRNA (SI00906220) (0 and 50 nM) and collected at the designated time point (24 h). After transfections, cells underwent standard immunofluorescence processing and were imaged. Each sample was compared to negative and positive controls, which were used to quantify the image results (*P < 0.05).