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. 2001 Apr 10;98(9):5110–5115. doi: 10.1073/pnas.091094198

Figure 2.

Figure 2

TI is a function of homologous DNA length. TI frequency vs. homologous DNA length is plotted for three different data sets. ▴, Five published gene knockouts in M. grisea strain Guy 11. MA, MAC1 (36); P, PMK1 (23); A, ABC1 (37); M, MPG1 (21); N = NUT1 (38). Published TI frequencies are 16%, 11%, 21%, 3%, and 22%, respectively. □, MAC1 TAGKO cosmid subjected to KpnI, EcoRI and SpeI, restriction digestion yielding, three MAC1 constructs with the same insertion but different homologous DNA lengths of 5.5 kb, 13.5 kb, and 20.2 kb, respectively. ○, Ten different TAGKO constructs created by IVT into a cosmid library pool.