Table 1.
Proteins identified from mouse liver microsomal preparations with 2DE-based strategy.
| Spot no. | IPIa | Protein name | Predicted TMD | Location |
|---|---|---|---|---|
| 90,91 | IPI00108939 | glyceraldehyde-3-phosphate dehydrogenase, spermatogenic | 0 | ER |
| 6 | IPI00111936 | UDP-glucuronosyltransferase 1-2 precursor, microsomal | 1 | ER |
| 145 | IPI00121833 | Acetyl-coenzyme A acyltransferase 1 | 0 | ER |
| 102 | IPI00622235 | Transitional endoplasmic reticulum ATPase | 0 | ER |
| 6,61,194 | IPI00122815 | Prolyl 4-hydroxylase, beta polypeptide | 0 | ER |
| 17 | IPI00123176 | Similar to glyceraldehyde-3-phosphate dehydrogenase, 37 kDa protein | 0 | ER |
| 134,135 | IPI00123342 | Hypoxia upregulated 1 | 1 | ER |
| 2 | IPI00129526 | Endoplasmin | 0 | ER |
| 139 | IPI00131459 | Nucleoside diphosphate kinase A | 0 | ER |
| 179 | IPI00132874 | Splice isoform 1 of monoglyceride lipase | 0 | ER |
| 163 | IPI00133522 | Protein disulfide-isomerase precursor | 0 | ER |
| 49 | IPI00134058 | Thioredoxin domain containing protein 4 precursor | 0 | ER |
| 108,145,65 | IPI00135284 | Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) ISOFORM 1 | 0 | ER |
| 147,148,149 | IPI00135686 | Mus musculus adult male kidney cDNA, RIKEN full-length enriched library, clone: 0610008 | 1 | ER |
| 174,178,179,183 | IPI00135726 | Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 0 | ER |
| 49,50 | IPI00163011 | Thioredoxin domain containing protein 5 precursor | 0 | ER |
| 137 | IPI00226993 | Thioredoxin | 0 | ER |
| 148 | IPI00229551 | ADAM4 | 1 | ER |
| 62,157,158,162 | IPI00230108 | Glucose-regulated protein, full insert sequence | 0 | ER |
| 148 | IPI00271869 | Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 0 | ER |
| 146,147,149,150,153 | IPI00273646 | Glyceraldehyde-3-phosphate dehydrogenase | 0 | ER |
| 187 | IPI00555023 | Glutathione S-transferase P 1 | 0 | ER |
| 144 | IPI00319652 | Glutathione peroxidase | 0 | ER |
| 84 | IPI00319992 | 78 kDa glucose-regulated protein precursor | 0 | ER |
| 153 | IPI00320208 | Elongation factor 1-beta | 0 | ER |
| 118 | IPI00323357 | Heat shock cognate 71 kDa protein | 0 | ER |
| 173 | IPI00323661 | Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 0 | ER |
| 145,201 | IPI00462605 | Similar to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | 0 | ER |
| 127 | IPI00469307 | Alpha-2-macroglobulin receptor-associated protein precursor | 0 | ER |
| 143,152,203 | IPI00480343 | 2700050F09Rik protein | 0 | ER |
| 162 | IPI00831714 | Leucine-rich repeat-containing protein 7 | 0 | ER (integral to membrane) |
| 149,150 | IPI00352124 | Flavin containing monooxygenase 5 | 1 | ER (integral to membrane) |
| 131 | IPI00132397 | GTP-binding protein SAR1b | 0 | ER (peripheral membrane protein) |
| 107 | IPI00227657 | Stromal cell-derived factor 2-like protein 1 precursor | 0 | ER lumen |
| 145 | IPI00123281 | Expressed sequence AA959742 | 1 | ER membrane |
| 7 | IPI00222496 | Protein disulfide isomerase-associated 6 | 1 | ER, membrane proteinb |
| 7 | IPI00112322 | UDP-glucuronosyltransferase 2B5 precursor | 1 | ER, membrane proteinsb |
| 156 | IPI00331322 | Microsomal glutathione S-transferase 1 | 3 | ER, outer membrane |
| 151 | IPI00319973 | Membrane-associated progesterone receptor component 1 | 1 | ER, membrane-bound |
| 152 | IPI00170316 | Multiple coagulation factor deficiency protein 2 homolog precursor | 0 | ER-Golgi intermediate compartment |
| 170 | IPI00408892 | RAS-related protein RAB-7 | 0 | Golgi, endosomes, lysosomes |
| 153 | IPI00123316 | Splice isoform 1 of tropomyosin 1 alpha chain | 0 | Cytoplasm |
| 116 | IPI00133456 | Senescence marker protein-30 | 0 | Cytoplasm |
| 89,129,130,151,152 | IPI00135085 | Heme-binding protein | 0 | Cytoplasm |
| 165 | IPI00109061 | Tubulin beta-4 chain homolog | 0 | Cytoplasmic |
| 88 | IPI00109073 | Tubulin beta-4 chain | 0 | Cytoplasmic |
| 105,138 | IPI00110753 | Tubulin alpha-1 chain | 0 | Cytoplasmic |
| 113,166,167,197,204 | IPI00110827 | Actin, alpha skeletal muscle | 0 | Cytoplasmic |
| 9,129,130,151.153,166, 167,197,198 |
IPI00110850 | Actin, cytoplasmic 1 | 0 | Cytoplasmic |
| 90 | IPI00114162 | Fatty acid-binding protein, epidermal | 0 | Cytoplasmic |
| 145 | IPI00116277 | T-complex protein 1, delta subunit | 0 | Cytoplasmic |
| 144 | IPI00117264 | DJ-1 protein | 0 | Cytoplasmic |
| 191,164,165 | IPI00117348 | Tubulin alpha-2 chain | 0 | Cytoplasmic |
| 137,138,164 | IPI00117350 | Tubulin alpha-4 chain | 0 | Cytoplasmic |
| 141,153,165,132 | IPI00117352 | Tubulin beta-5 chain | 0 | Cytoplasmic |
| 126 | IPI00117914 | Arginase 1 | 0 | Cytoplasmic |
| 152 | IPI00120532 | 21 kDa protein | 0 | Cytoplasmic |
| 107,108,139,143,182 | IPI00125489 | 44 KD protein (Argininosuccinate synthase) | 0 | Cytoplasmic |
| 191 | IPI00626790 | Glutamine synthetase | 0 | Cytoplasmic |
| 176,182,194 | IPI00130950 | Betaine-homocysteine S-methyltransferase | 0 | Cytoplasmic |
| 99 | IPI00131204 | UDP-glucose pyrophosphorylase 2 | 0 | Cytoplasmic |
| 204 | IPI00136929 | Gamma actin-like protein | 0 | Cytoplasmic |
| 101,132 | IPI00169463 | Tubulin beta-2C Chain | 0 | Cytoplasmic |
| 202,133 | IPI00221400 | Alcohol dehydrogenase A chain | 0 | Cytoplasmic |
| 89 | IPI00221528 | Actin, cytoplasmic type 5 homolog | 0 | Cytoplasmic |
| 168 | IPI00221890 | Carbonic anhydrase III | 0 | Cytoplasmic |
| 202,133 | IPI00317740 | Guanine nucleotide-binding protein beta subunit 2-like 1 | 0 | Cytoplasmic |
| 159 | IPI00331174 | T-complex protein 1, eta subunit | 0 | Cytoplasmic |
| 154 | IPI00338039 | Tubulin, beta 2 | 0 | Cytoplasmic |
| 141 | IPI00348094 | Predicted: similar to tubulin M beta 1 | 0 | Cytoplasmic |
| 136 | IPI00404011 | Microtubule-associated protein | 0 | Cytoplasmic |
| 153 | IPI00421223 | Tropomyosin alpha 4 chain | 0 | Cytoplasmic |
| 194,195 | IPI00457825 | Similar to argininosuccinate synthase (Citrulline-aspartate ligase) | 0 | Cytoplasmic |
| 60 | IPI00462072 | Similar to alpha enolase (2-phospho-D-glycerate hydro-lyase) | 0 | Cytoplasmic |
| 178 | IPI00467066 | Glycine N-methyltransferase | 0 | Cytoplasmic |
| 63,109 | IPI00467833 | Triosephosphate isomerase | 0 | Cytoplasmic |
| 153 | IPI00605380 | Similar to tubulin alpha-2 chain (Alpha-tubulin 2) | 0 | Cytoplasmic |
| 162 | IPI00123313 | Ubiquitin-activating enzyme E1 1 | 0 | Cytoplasmic and nuclear |
| 64 | IPI00420745 | Proteasome subunit, alpha type 2, full insert sequence | 0 | Cytoplasmic and nuclear |
| 145 | IPI00320165 | Oxidoreductase HTATIP2 | 0 | Cytoplasmic and nuclear |
| 153 | IPI00117978 | Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor | 1 | Mitochondrial inner membrane |
| 19 | IPI00109167 | NADH-ubiquinone oxidoreductase 24 kDa subunit | 0 | Mitochondrial inner membrane |
| 158 | IPI00111885 | Ubiquinol-cytochrome-c reductase complex core protein I, mitochondrial precursor | 0 | Mitchondrial inner membrane |
| 175 | IPI00121322 | Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial precursor | 0 | Mitchondrial inner membrane |
| 196 | IPI00128023 | NADH-ubiquinone oxidoreductase 49 kDa subunit, mitochondrial precursor | 0 | Mitchondrial inner membrane |
| 134 | IPI00111908 | Predicted: carbamoyl-phosphate synthetase 1 | 0 | Mitochondrial |
| 145 | IPI00114840 | Endonuclease G, mitochondrial precursor | 0 | Mitochondrial |
| 70 | IPI00331555 | 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial precursor | 0 | Mitochondrial |
| 94,95 | IPI00115607 | Trifunctional enzyme beta subunit, mitochondrial precursor | 0 | Mitochondrial |
| 145 | IPI00115824 | NipSnap1 protein | 0 | Mitochondrial |
| 22 | IPI00116154 | Cytochrome c oxidase, subunit vb, full insert sequence | 0 | Mitochondrial |
| 15,146,147,148,149,100 | IPI00118986 | ATP synthase oligomycin sensitivity conferral protein, mitochondrial precursor | 0 | Mitochondrial |
| 127 | IPI00119138 | Ubiquinol-cytochrome-c reductase complex core protein 2, mitochondrial precursor | 0 | Mitochondrial |
| 147,148 | IPI00120984 | NADH-ubiquinone oxidoreductase 19 kDa subunit | 0 | Mitochondrial |
| 137 | IPI00129516 | Ubiquinol-cytochrome c reductase complex 11 kDa protein, mitochondrial precursor | 0 | Mitochondrial |
| 93,99,100,192,203 | IPI00130280 | ATP synthase alpha chain, mitochondrial precursor | 0 | Mitochondrial |
| 149,150 | IPI00132217 | Tetratricopeptide repeat protein 11 | 1 | Mitochondrial |
| 150,151 | IPI00132390 | NADH-ubiquinone oxidoreductase B15 subunit | 1 | Mitochondrial |
| 101,132,137,141,153 | IPI00170093 | NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial precursor | 0 | Mitochondrial |
| 92,93,94,95,96 | IPI00223092 | Hydroxyacyl-coenzyme A dehydrogenase/3-ketoacyl-coenzyme A | 0 | Mitochondrial |
| 142,143,152 | IPI00230507 | ATP synthase D chain, mitochondrial | 0 | Mitochondrial |
| 162 | IPI00308882 | NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor | 0 | Mitochondrial |
| 149 | IPI00344004 | 13 KDa differentiation-associated protein | 0 | Mitochondrial |
| 145,146 | IPI00420718 | Hydroxymethylglutaryl-CoA synthase, mitochondrial precursor | 0 | Mitochondrial |
| 51 | IPI00308885 | 60 kDa heat shock protein, mitochondrial | 0 | Mitochondrial |
| 153 | IPI00462250 | Similar to adenine nucleotide translocase | 3 | Mitochondrial |
| 85,165,167,203 | IPI00468481 | ATP synthase beta chain, mitochondrial precursor | 0 | Mitochondrial |
| 147 | IPI00117281 | Phospholipid hydroperoxide glutathione peroxidase, mitochondrial precursor | 0 | Mitochondrial and cytoplasmic |
| 169 | IPI00133240 | Ubiquinol-cytochrome c reductase iron-sulfur subunit, mitochondrial precursor | 0 | Mitochondrial inner membrane |
| 200,201 | IPI00230351 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial precursor | 0 | Mitochondrial inner membrane |
| 174 | IPI00132042 | Pyruvate dehydrogenase E1 component beta subunit, mitochondrial precursor | 0 | Mitochondrial matrix |
| 156 | IPI00315794 | Cytochrome b5 outer mitochondrial membrane isoform precursor | 1 | Mitochondrial outer membrane |
| 65,109,144,170,176,100 | IPI00134746 | Argininosuccinate synthase | 0 | mitochondrion |
| 145,146 | IPI00338536 | Succinate dehydrogenase [ubiquinone] iron-sulfur protein, mitochondrial precursor | 0 | Mitochondrion |
| 112 | IPI00122547 | Voltage-dependent anion-selective channel protein 2 | 0 | Mitochondrion outer membrane |
| 147 | IPI00131186 | Splice isoform 2 of transcription factor BTF3 | 0 | Nuclear |
| 149 | IPI00317794 | Nucleolin | 0 | Nuclear |
| 53 | IPI00331146 | UMP-CMP kinase | 0 | Nuclear |
| 150 | IPI00458856 | Similar to ZNF91L isoform 1 | 0 | Nuclear |
| 6 | IPI00461822 | E1A binding protein p300 | 0 | Nuclear |
| 55 | IPI00126172 | RIKEN cDNA 4931406C07, PTD012 homolog | 0 | Nuclear |
| 150,151 | IPI00113241 | 40S ribosomal protein S19 | 0 | Ribosome |
| 104 | IPI00116908 | Similar to 40 s ribosomal protein S12 | 0 | Ribosome |
| 147 | IPI00849793 | 60S ribosomal protein L12 | 0 | Ribosome |
| 81,127,145 | IPI00125971 | 26S protease regulatory subunit S10B | 0 | Ribosome |
| 199 | IPI00123604 | 40S ribosomal protein SA | 0 | Ribosome |
| 125 | IPI00135640 | 26S protease regulatory subunit 8 | 0 | Ribosome |
| 149,150,151 | IPI00139780 | 60S ribosomal protein L23 | 0 | Ribosome |
| 149,150 | IPI00222546 | 60S ribosomal protein L22 | 0 | Ribosome |
| 145 | IPI00314950 | 60S acidic ribosomal protein P0 | 0 | Ribosome |
| 149 | IPI00322562 | 40S ribosomal protein S14 | 0 | Ribosome |
| 146 | IPI00331092 | 40S ribosomal protein S4, X isoform | 0 | Ribosome |
| 149,150 | IPI00331461 | 60S ribosomal protein L11 | 0 | Ribosome |
| 188 | IPI00351894 | Similar to ribosomal protein | 0 | Ribosome |
| 148 | IPI00849793 | 60S ribosomal protein L12 | 0 | Ribosome |
| 149 | IPI00465880 | 40S ribosomal protein S17 | 0 | Ribosome |
| 199 | IPI00123604 | 40S ribosomal protein SA | 0 | Ribosome |
| 4,11 | IPI00121209 | Apolipoprotein A-I precursor | 0 | Secreted |
| 149 | IPI00121837 | Ribonuclease 4 precursor | 1 | Secreted |
| 12 | IPI00122429 | Plasma retinol-binding protein precursor | 0 | Secreted |
| 163 | IPI00123920 | Alpha-1-antitrypsin 1–3 precursor | 0 | Secreted |
| 163 | IPI00123924 | Alpha-1-antitrypsin 1–4 precursor | 0 | Secreted |
| 163 | IPI00123927 | Alpha-1-antitrypsin 1–5 precursor | 0 | Secreted |
| 162 | IPI00128484 | Hemopexin precursor | 1 | Secreted |
| 3,117,118 | IPI00131695 | Serum albumin precursor | 0 | Secreted |
| 98 | IPI00139788 | Serotransferrin precursor | 0 | Secreted |
| 199 | IPI00323571 | Apolipoprotein E precursor | 0 | Secreted |
| 135,136 | IPI00377351 | Apolipoprotein A-IV precursor | 0 | Secreted |
| 163 | IPI00406302 | Alpha-1-antitrypsin 1-1 precursor | 0 | Secreted |
| 100,155,156 | IPI00466399 | 21 kDa protein | 0 | Secreted |
| 156 | IPI00480401 | Major urinary protein 1 precursor | 0 | Secreted |
| 122 | IPI00130661 | Tripeptidyl-peptidase I precursor | 0 | Secreted (lysosomal) |
| 101 | IPI00115302 | Branched chain ketoacid dehydrogenase E1, beta polypeptide | 0 | Membrane |
| 197 | IPI00120716 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 1 | 0 | Membrane |
| 21,137 | IPI00120719 | Cytochrome c oxidase, subunit va, full insert sequence | 0 | Membrane |
| 125 | IPI00124790 | Polyposis locus protein 1-like 1 | 3 | Membrane |
| 129 | IPI00132076 | Catechol O-methyltransferase | 1 | Membrane |
| 130,142 | IPI00138406 | Ras-related protein Rap-1A | 0 | Membrane |
| 174 | IPI00162780 | Guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 2 | 0 | Membrane |
| 88,154 | IPI00230113 | Cytochrome b5 | 1 | Membrane |
| 199,200 | IPI00353727 | Annexin A4 | 0 | Membrane |
| 110 | IPI00117416 | Neighbor of COX4 | 0 | Unknown |
| 143 | IPI00121271 | Hypothetical S-adenosyl-L-methionine-dependent methyltransferases structure containing protein | 0 | Unknown |
| 144,108 | IPI00267667 | RIKEN cDNA 6330409N04, CLLL6 protein homolog | 0 | Unknown |
| 101 | IPI00269613 | Eukaryotic translation initiation factor 3 subunit 2 | 0 | Unknown |
| 149,150 | IPI00307837 | 51 kDa protein | 0 | Unknown |
| 203 | IPI00318204 | Sid6061p | 0 | Unknown |
| 105 | IPI00273646 | Similar to glyceraldehyde-3-phosphate dehydrogenase | 0 | Unknown |
| 189,190,194 | IPI00626790 | Glutamine synthetase | 0 | Unknown |
| 50 | IPI00345842 | 86 KDa PROTEIN | 0 | Unknown |
| 51 | IPI00350780 | 45 kDa protein | 0 | Unknown |
| 133 | IPI00381231 | 77 KDa protein | 0 | Unknown |
| 144 | IPI00923085 | Probable ubiquitin-conjugating enzyme E2 FLJ25076 homolog | 0 | Unknown |
| 146,147,150,173 | IPI00460295 | 44 KDa protein | 0 | Unknown |
| 156 | IPI00330913 | Major urinary protein 26 | 0 | Unknown |
| 59 | IPI00467988 | 169 kDa protein | 0 | Unknown |
| 100,155,156 | IPI00469517 | 21 kDa protein | 0 | Unknown |
| 149 | IPI00130554 | Splice isoform 1 of SNARE-associated protein Snapin | 0 | Vesicular membrane |
| 101,127,134 | IPI00131366 | Keratin, type II cytoskeletal 6B | 0 | Sarcolemma |
| 83,106,107 | IPI00121788 | Peroxiredoxin 1 | 0 | Microbody |
| 101,139 | IPI00348328 | Keratin Kb40 | 0 | Intermediate filament |
| 156 | IPI00137414 | Left-right dynein | 0 | Cilium |
aESI-Q-TOF identification, subcellular location are given for each ID number.
bThis protein is nonmembrane associated according to the annotation in the Swiss-Prot database but has one predicted TMD.