Table 2.
Proteins identified from Na2CO3-extracted mouse liver microsomal membrane preparations with 1DE-based strategy.
| Bands no. | Accession no. | Protein name | Predicted TMD | GRAVY score | PI value | Subcellular location |
|---|---|---|---|---|---|---|
| 9 | IPI00112322 | UDP-glucuronosyltransferase 2B5 precursor | 1 | −0.031 | 7.94 | ER |
| 9 | IPI00127223 | UDP glucuronosyltransferase 2 family, polypeptide B36 | 1 | −0.036 | 8.47 | ER |
| 9 | IPI00222496 | Protein disulfide-isomerase A6 | 1 | −0.292 | 5.05 | ER |
| 8 | IPI00417182 | UDP-glycosyltransferase 1 family polypeptide A5 | 1 | 0.044 | 8.33 | ER |
| 9 | IPI00116572 | Cytochrome P450, family 2, subfamily d, polypeptide 9 | 2 | −0.043 | 6.37 | ER |
| 15 | IPI00113655 | 40S ribosomal protein S6 | 0 | −0.918 | 10.68 | ER |
| 5 | IPI00129526 | Endoplasmin precursor (ER protein 99,94 kDa glucose-regulated protein) | 0 | −0.72 | 4.74 | ER |
| 13 | IPI00130985 | Short-chain dehydrogenase CRAD2 | 0 | 0.026 | 8.35 | ER |
| 6 | IPI00222809 | Similar to GDH/6PGL endoplasmic bifunctional protein | 0 | −0.18 | 6.61 | ER |
| 8 | IPI00230108 | Glucose-regulated protein, full insert sequence | 0 | −0.479 | 5.78 | ER |
| 10,11 | IPI00317356 | Paraoxonase 1 | 0 | −0.01 | 5.02 | ER |
| 7 | IPI00319992 | 78 kDa glucose-regulated protein precursor | 0 | −0.481 | 5.07 | ER |
| 13 | IPI00121079 | NADH-cytochrome b5 reductase 3 | 0 | −0.203 | 8.56 | ER, membrane bound |
| 9 | IPI00123964 | Cytochrome P450 2A5 | 1 | −0.203 | 9.23 | ER, membrane bound |
| 9 | IPI00114779 | Cytochrome P450 2C38 | 0 | −0.147 | 8.69 | ER, membrane bound |
| 17 | IPI00331322 | Microsomal glutathione S-transferase 1 | 3 | 0.14 | 9.67 | ER and mitochondrial outer membrane |
| 17 | IPI00119766 | Cis-retinol androgen dehydrogenase 1 | 0 | 0.005 | 9.25 | ER lumen |
| 8 | IPI00134691 | UDP-glucuronosyltransferase 1-1 precursor, microsomal | 2 | 0.087 | 8.87 | ER, integral to plasma membrane |
| 8 | IPI00128287 | Cytochrome P450 1A2 | 1 | −0.203 | 8.92 | ER, membrane bound |
| 10 | IPI00136910 | Cytochrome P450 2D11 | 2 | −0.009 | 6.15 | ER, membrane bound |
| 9 | IPI00308328 | Cytochrome P450 2F2 | 1 | −0.135 | 7.74 | ER, membrane bound |
| 9,10 | IPI00323908 | Cytochrome P450 2D10 | 2 | −0.073 | 6.16 | ER, membrane bound |
| 7 | IPI00112549 | Long-chain-fatty-acid-CoA ligase 1 | 1 | −0.045 | 6.81 | ER, type III membrane protein |
| 8 | IPI00133522 | Protein disulfide-isomerase precursor | 0 | −0.386 | 4.79 | ER |
| 9 | IPI00116572 | Cytochrome P450 2D9 | 0 | −0.063 | 5.93 | ER, membrane bound |
| 5,6 | IPI00119618 | Calnexin precursor | 1 | −0.875 | 4.5 | ER, type I membrane protein |
| 1,10,12,14,15 | IPI00319973 | Membrane-associated progesterone receptor component 1 | 1 | −0.616 | 4.57 | ER, membrane bound |
| 8 | IPI00132475 | Protein ERGIC-53 | 1 | −0.545 | 5.92 | ER-Golgi intermediate compartment (ERGIC), type I membrane protein |
| 8,17 | IPI00109061 | Tubulin beta-4 chain homolog | 0 | −0.406 | 4.78 | Cytoplasmic |
| 10 | IPI00110827 | Actin, alpha skeletal muscle | 0 | −0.232 | 5.23 | Cytoplasmic |
| 10,12,14 | IPI00110850 | Actin, cytoplasmic 1 | 0 | −0.2 | 5.29 | Cytoplasmic |
| 1,2,3 | IPI00111908 | Carbamoyl-phosphate synthase | 0 | −0.12 | 6.42 | Cytoplasmic |
| 1,8,9,13 | IPI00117348 | Tubulin alpha-2 chain | 0 | −0.23 | 4.94 | Cytoplasmic |
| 9,10,11,12,13 | IPI00117914 | Arginase 1 | 0 | −0.187 | 6.52 | Cytoplasmic |
| 17 | IPI00120451 | Fatty acid-binding protein, liver | 0 | −0.409 | 8.59 | Cytoplasmic |
| 9 | IPI00129028 | Similar to tubulin, alpha 3C isoform 1 | 0 | −0.204 | 4.98 | Cytoplasmic |
| 1–11,13,17 | IPI00130950 | Betaine-homocysteine S-methyltransferase | 0 | −0.36 | 8.01 | Cytoplasmic |
| 1,4,6,10,11,14,15 | IPI00134746 | Argininosuccinate synthase | 0 | −0.361 | 8.36 | Cytoplasmic |
| 3,4 | IPI00114710 | Pyruvate carboxylase, mitochondrial precursor | 0 | −0.173 | 6.25 | Mitochondrial |
| 17 | IPI00553333 | Hemoglobin subunit beta-1 | 0 | 0.092 | 7.13 | Mitochondrial |
| 9 | IPI00134809 | Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex | 0 | −0.171 | 9.1 | Mitochondrial |
| 17 | IPI00117978 | Cytochrome c oxidase subunit IV isoform 1, mitochondrial precursor | 1 | −0.412 | 9.25 | Mitochondrial inner membrane |
| 15,16 | IPI00315794 | Cytochrome b5 outer mitochondrial membrane isoform precursor | 1 | −0.602 | 4.79 | Mitochondrial outer membrane |
| 13 | IPI00321718 | Prohibitin-2 | 0 | −2.58 | 9.83 | Mitochondrial, cytoplasmic, nuclear |
| 13 | IPI00122547 | Voltage-dependent anion-selective channel protein 2 | 0 | −0.223 | 7.44 | Outer mitochondrial Membrane |
| 17 | IPI00114559 | Histone H2A type 1 | 0 | −0.572 | 11.22 | Nuclear |
| 16,17 | IPI00114642 | Histone H2B F | 0 | −0.762 | 10.32 | Nuclear |
| 8 | IPI00387318 | Cell cycle control protein 50A | 2 | −0.331 | 8.58 | Membrane |
| 15 | IPI00113849 | Splice isoform 2 of cell division control protein 42 homolog | 0 | −0.157 | 6.16 | Membrane |
| 13 | IPI00122549 | Splice isoform Pl-VDAC1 of voltage-dependent anion-selective channel protein 1 | 0 | −0.334 | 8.55 | Membrane |
| 15 | IPI00127408 | Ras-related C3 botulinum substrate 1 | 0 | −0.101 | 8.77 | Membrane |
| 15 | IPI00138406 | Ras-related protein Rap-1A | 0 | −0.375 | 6.39 | Membrane |
| 6 | IPI00116921 | Scavenger receptor class B member 1 | 2 | 0.073 | 8.29 | Integral membrane protein |
| 1 | IPI00121985 | Splice Isoform 1 of solute carrier organic anion transporter family, member 1B2 | 12 | 0.172 | 8.95 | Integral membrane protein |
| 9 | IPI00124830 | Integrin-associated protein precursor | 5 | 0.563 | 8.58 | Integral membrane protein |
| 14,15 | IPI00131176 | Cytochrome c oxidase subunit 2 | 2 | 0.27 | 4.6 | Integral membrane protein |
| 1 | IPI00132604 | Secretedretory carrier-associated membrane protein 3 | 4 | 0.028 | 7.55 | Integral membrane protein |
| 1 | IPI00135701 | Solute carrier organic anion transporter family, member 1A1 | 11 | 0.244 | 8.58 | Integral membrane protein |
| 1 | IPI00311682 | Sodium/potassium-transporting ATPase alpha-1 chain precursor | 10 | 0.002 | 5.3 | Integral membrane protein |
| 6 | IPI00331214 | Platelet glycoprotein IV | 2 | −0.053 | 8.61 | Integral membrane protein |
| 2 | IPI00119063 | Prolow-density lipoprotein receptor-related protein 1 | 1 | −0.502 | 5.17 | Integral to membrane |
| 1,12,16,17 | IPI00124790 | Polyposis locus protein 1-like 1 | 3 | 0.375 | 6.82 | Integral to membrane |
| 10 | IPI00129677 | Asialoglycoprotein receptor major subunit | 1 | −0.66 | 5.99 | Integral to membrane |
| 17 | IPI00467119 | Camello-like protein 1 | 1 | 0.302 | 9.61 | Integral to membrane |
| 5,8 | IPI00316329 | Keratin, type II cytoskeletal 1 | 0 | −0.588 | 8.2 | Intermediate filament |
| 10,11,12 | IPI00108844 | Cation-dependent mannose-6-phosphate receptor precursor | 1 | −0.168 | 5.24 | Type I membrane protein |
| 9 | IPI00109998 | H-2 class I histocompatibility antigen, D-B alpha chain precursor | 1 | −0.508 | 6.28 | Type I membrane protein |
| 4 | IPI00120245 | Integrin alpha-V precursor | 1 | −0.246 | 5.46 | Type I membrane protein |
| 3,4 | IPI00121190 | Epidermal growth factor receptor precursor | 2 | −0.316 | 6.46 | Type I membrane protein |
| 2 | IPI00126186 | Macrophage mannose receptor 1 precursor | 1 | −0.5 | 6.47 | Type I membrane protein |
| 5 | IPI00134549 | Splice isoform LAMP-2A of lysosome-associated membrane glycoprotein 2 precursor | 1 | −0.036 | 7.05 | Type I membrane protein |
| 13 | IPI00312018 | Malectin | 1 | −0.203 | 5.73 | Type I membrane protein |
| 3 | IPI00312063 | Low-density lipoprotein receptor precursor | 1 | −0.391 | 4.88 | Type I membrane protein |
| 16 | IPI00466570 | Transmembrane emp24 domain-containing protein 10 | 2 | −0.169 | 6.25 | Type I membrane protein |
| 4 | IPI00108535 | Carcinoembryonic antigen-related cell adhesion molecule 1 | 1 | −0.302 | 5.35 | Type I membrane protein |
| 5 | IPI00310059 | Polymeric-immunoglobulin receptor precursor | 1 | −0.499 | 5.26 | Type I membrane protein also secreted |
| 9 | IPI00121550 | Sodium/potassium-transporting ATPase beta-1 chain | 1 | −0.55 | 8.83 | Type II membrane protein |
| 2,3 | IPI00134585 | Glutamyl aminopeptidase | 1 | −0.344 | 5.28 | Type II membrane protein |
| 10 | IPI00307966 | ADP-ribosyl cyclase 1 | 1 | −0.106 | 8.64 | Type II membrane protein |
| 3 | IPI00319509 | Aminopeptidase N | 1 | −0.277 | 5.62 | Type II membrane protein |
| 3 | IPI00458003 | Ectonucleotide pyrophosphatase/phosphodiesterase 3 | 1 | −0.346 | 6.13 | Unknown |
| 9 | IPI00409409 | CD1D1 protein | 1 | −0.178 | 9.22 | Unknown |
| 7 | IPI00621548 | NADPH-cytochrome P450 reductase | 1 | −0.463 | 5.37 | Unknown |
| 9 | IPI00321644 | Cytochrome P450 2D26 | 2 | −0.105 | 6.16 | Unknown |
| 1 | IPI00127016 | Hydroxysteroid 17-beta dehydrogenase 6 | 0 | −0.075 | 8.63 | Unknown |
| 16 | IPI00221721 | Hypothetical krab box containing protein, full insert sequence | 0 | −0.142 | 9.84 | Unknown |
| 8 | IPI00224073 | Hypothetical peptidase family M20/M25/M40 containing protein, full insert sequence | 0 | −0.01 | 5.99 | Unknown |
| 16 | IPI00228379 | Ferritin light chain 2 | 0 | −0.479 | 6.37 | Unknown |
| 17 | IPI00266842 | 17 kDa protein | 0 | −0.668 | 10.48 | Unknown |
| 15 | IPI00379258 | Similar to ferritin light chain 2 | 0 | −0.454 | 8.51 | Unknown |
| 3 | IPI00405742 | Plexin B2 | 0 | −0.3 | 5.67 | Unknown |
| 2 | IPI00408258 | Structure-specific endonuclease subunit SLX4 | 0 | −0.714 | 5.33 | Unknown |
| 11 | IPI00462251 | Hypothetical protein LOC72792 isoform 1 | 0 | −0.429 | 5.82 | Unknown |
| 15 | IPI00605814 | Similar to Ferritin light chain 1 | 0 | −0.358 | 6.42 | Unknown |
| 10 | IPI00131366 | Keratin, type II cytoskeletal 6B | 0 | −0.488 | 8.32 | Sarcolemma |
| 10 | IPI00322209 | Keratin, type II cytoskeletal 8 | 0 | −0.602 | 5.7 | Sarcolemma |
| 8 | IPI00853991 | Similar to VH coding region | 0 | −0.102 | 5.31 | Secreted |
| 10 | IPI00126458 | MRNA | 1 | −0.55 | 5.66 | MHC class I protein complex |
| 15 | IPI00121788 | Peroxiredoxin 1 | 0 | −0.221 | 8.26 | Microbody |