Table 5.
| Current study | Ref. [19] | Ref. [18] | Ref. [20] | |
|---|---|---|---|---|
| Species | Mouse | Mouse | Mouse | Rat |
| Sample | Liver microsome | Liver microsome | Liver microsome | ER, Golgi |
| Pretreatment | None | Phenobarbital | Phenobarbital or 3-methylcholanthrene | None |
| Sample preparation | Subfractionated by differential ultra-centrifugation + sucrose gradient centrifugation + Na2CO3 | Subfractionated by differential ultra-centrifugation | Differential ultracentrifugation | Subfractionated by differential ultra-centrifugation + density gradient centrifugation |
| Protein separation | 2DE, 1DE | 1DE | 2DE | 1DE |
| 1D/2D-Gel Stain | Coomassie brilliant blue (2DE; 1DE) | — | Silver stain | — |
| Protein identification | MS/MS | MS/MS | PMF | MS/MS |
| Protein spots on 2D-Gel | 514 | — | 1100 | — |
| Proteins identified in 2D-Gel | 183 | — | 139 | — |
| Proteins identified in 1DE | 99 | 519 | — | 832 (ER) |
| Proteins identified in 2-D LC | — | 1410 | — | — |
| Proteins identified in 3-D LC | — | 3703 | — | — |
| Total identified proteins | 259 | 4142 | Unspecified | 832 (ER) |
| Membrane proteins | 2DE: 41 (41/183 = 22%) 1DE: 54 (54/99 = 55%) Total: 62 (62/259 = 24%) |
1DE: 138 (138/519 = 27%) 2-D LC: 259 (259/1410 = 21%) 3-D LC: 659 (659/3703 = 18%) |
Unspecified | 183 (183/832 = 22%) |
| Protein superfamily | ||||
| P450 family members | 10 | 29 | 2 | 11 |
| Ribosomal proteins | 13 | 16 | Unspecified | 45 |
| UDP glycosyltransferases, UGTs | 6 | 8 | Unspecified | 3 |
| Tubulins | 11 | 5 | Unspecified | 2 |
| Short-chain dehydrogenase/reductase | 32 | 9 | Unspecified | 56 |
| Protein disulfide isomerase | 2 | 4 | Unspecified | 1 |