Skip to main content
. 2012 Apr;91(4):667–675. doi: 10.1189/jlb.1011510

Figure 5. Effect of host-derived IL-10 on Treg-mediated suppression of GVHD.

Figure 5.

(A) B6(WT) recipients were irradiated (10 Gy) on Day −1 and transplanted 5 × 106 syngeneic B6 BM (▾; n=9), B6-IL-10KO recipients were irradiated (10 Gy) on Day −1 and transplanted allogeneic C3H.SW 5 × 106 TCD BM + 0.2 × 106 CD8 T cells (■; n=12), or C3H.SW 5 × 106 TCD BM + 0.2 × 106 CD8 T cells + 0.1 × 106 CD4+CD25+ T cells (□; n=12). Survival was monitored daily. Data from two similar experiments are combined and shown. ■ versus □, P = 0.0136. (B) B6(WT) BM chimera recipients [B6→Ly5.2] were irradiated (9 Gy) on Day −1 and transplanted 5 × 106 syngeneic B6 BM (▾; n=5), allogeneic C3H.SW 5 × 106 TCD BM + 0.2 × 106 CD8 T cells (●; n=6), or C3H.SW 5 × 106 TCD BM + 0.2 × 106 CD8 T cells + 0.1 × 106 CD4+CD25+ T cells (○; n=5). B6-IL-10KO BM chimera recipients [IL-10KO→Ly5.2] were irradiated (9 Gy) on Day −1 and transplanted allogeneic C3H.SW 5 × 106 TCD BM + 0.2 × 106 CD8 T cells (■; n=6) or C3H.SW 5 × 106 TCD BM + 0.2 × 106 CD8 T cells + 0.1 × 106 CD4+CD25+ T cells (□; n=6). Survival was monitored daily. Data from one of two similar experiments are shown. ● versus ○, P = 0.0013; ■ versus □, P = 0.0005.