Table 1. Tat effects on respiratory chain complex activities.
Malonate-sensitive SCCR (CII-III)
|
Antimycin-sensitive QCCR (CIII)
|
Rotenone-sensitive NCCR (CI+III)
|
COX (CIV)
|
|
---|---|---|---|---|
Brain homogenate | (nmol/min per mg protein) | |||
Co. | 16 | 48 | 20 | 63 |
Tat 1 μM | 15 | 45 | 20 | 2 |
Buffer (5 μl)a | — | — | — | 63 |
Abbreviations: SCCR, succinate cytochrome c reductase; QCCR, quinol cytochrome c reductase; NCCR, NADH cytochrome c reductase.
Buffer refers to Tat solution buffer.
Values are means of triplicate experiments. Variability was <5%.
Activities of the various segments of the respiratory chain (succinate cytochrome c and NADH cytochrome c reductases) and of isolated complexes (decylubiquinol cytochrome c reductase and cytochrome c oxidase) were spectrophotometrically measured as described under Materials and Methods.