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Western blot using an antibody specific for the extracellular domain of VEGFR1. An increased band was found around 75 kDa (corresponding to sFlt-1) in the Flt morpholino group. When an antibody specific for the C terminus of VEGFR1 was used, a decreased 80-kDa band (corresponding to mFlt-1) was seen in the Flt morpholino group.
