Figure 3.

In vitro conversion of CD4⁺ T lymphocytes into functionally active antigen-specific Treg. Efficiency of CD4⁺CD25⁺FoxP3⁺ conversion in transwell cultures. Shown are the results of three independent experiments in which splenocytes from naive mice were seeded on the top of the transwell and MSCs were plated on the bottom part of the transwell with IRBP 1–20 (MSC⁺ IRBP 1–20) or without IRBP 1–20 (MSC) peptide added to the medium. The IRBP 1–20 bar refers to transwells in which splenocytes were seeded on the top part of the transwell, the IRBP peptide was added to the culture medium, and the bottom part of the transwell remained empty. *P < 0.0002 comparing the percentage of Treg generated in the presence of MSCs in vitro stimulated with the peptide and the other conditions.