Figure 4.

Effect of α-MHC class II or α-TGFβ antibodies on in vitro conversion of CD4⁺ T lymphocytes into functionally active antigen-specific Treg. (A) Percentage of CD4⁺CD25⁺FoxP3⁺ T lymphocytes isolated from the top portion of transwell experiments in which splenocytes from naive mice were seeded. *P < 0.001 comparing the percentage of Treg generated in the presence of MSCs and peptide (MSC⁺IRBP) with all the other conditions. **P < 0.001 comparing wells in which Treg were generated in the presence of MSC, IRBP peptide, and α-IA^b blocking antibodies (MSC⁺IRBP⁺α-IA^b) with all the other conditions. (B) TGFβ expression by MSCs isolated from transwell plates in the absence (MSC) or presence of α-TGFβ blocking antibodies (MSC⁺α-TGFβ). Shown are the compiled results of three independent experiments. *P < 0.001 comparing the two different conditions. (C) Proliferation of T lymphocytes isolated from uveitic mice to the immunizing antigen in the presence or absence of CD4⁺CD25⁺FoxP3⁺ T lymphocytes (Treg) isolated from the transwell experiments. Shown are compiled results of three independent experiments. *P < 0.001 comparing the proliferation observed in any experimental condition with the baseline value (medium). Bars indicate mean + SD.