Figure 4.

Exposure to topographically patterned surfaces decreases transformation of rabbit corneal fibroblasts to myofibroblasts. (A) Representative images of stromal cells cultured on indicated substrates and indicated amounts of exogenous TGFβ added to growth medium. Green signal: αSMA; blue: DAPI. (B) Graph depicting quantification of αSMA-positive cells. Cells grown on the 1400 nm pitch had fewer than 50% the number of transformed cells than those grown on planar surfaces when exposed to 10 ng/mL TGFβ. Error bars indicate SD. *P < 0.05.