Figure 2.

Effect on wound healing of CTCF overexpression. (A) Effect of overexpressing CTCF on HCE cell wound closures. (B) Significant enhancement of the wound closure rate by overexpression of CTCF. (C) Effect of overexpressed CTCF on EGF-induced wound healing. (D) Expression level of CTCF protein in CTCF cDNA-transfected HCE cells. HCE cells were transfected with both the vector plasmid as a control and the full-length cDNA encoding CTCF by lipofection. For EGF-induced experiments, cells were cultured in a serum-deprived condition (starvation) for at least 24 hours. *Significant difference between vector and CTCF cDNA–transfected cells (P < 0.05, n = 4).