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Effect of suppressed CTCF expression on EGF-induced cytoskeleton reorganization. The effect of CTCF mRNA knockdown on EGF-induced cytoskeleton reorganization was determined in growth factor–deprived HTCE cells infected with NS- and CTCF-specific shRNAs by immunostaining with FA, vinculin, and FA+Vin antibodies. Immunostained cells were imaged by an inverted microscope with a 60× TIRF lens and digital CCD camera. Scale bar, 20 μm.
