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. 2011 Dec 22;44(3):214–224. doi: 10.3858/emm.2012.44.3.016

Figure 2.

Figure 2

NM23-H2 over-expression in HCC. (A) Specific immunoreactivity of NM23-H1 or NM23-H2 in cell lysates. Cells were transiently transfected with expression plasmids of NM23-H1 or NM23-H2. PC, parent cells; VC, vector control. (B) NM23-H2 over-expression in HCCs and surrounding matrix tissues according to Edmondson-Steiner grading (GI to GIII). (C) NM23-H1 was over-expressed in HCCs but not in surrounding matrix tissues. (D) Immunofluorescence assay showing ectopic expression is mainly localized in the cytoplasm and to a lesser extent in the nucleus of HEK293T and HepG2 cells. Hoechst 33258 staining of nuclei (blue). Immunoreactivity of NM23-H2 (red) is perfectively merged with GFP and NM23-H2 fusion proteins; bar, 20 µm.