Table 1.
Primary and secondary antibodies for immunohistchemistry
| primary and secondary antibody | working dilution | type | source |
|---|---|---|---|
| green fluorescent protein (GFP) | 1:1000 | chick polyclonal | Aves |
| green fluorescent protein (GFP) | 1:1000 | rabbit polyclonal | Abcam |
| Insulin | 1:500 | guinea pig polyclonal | Dako |
| Glucagon | 1:2000 | mouse monoclonal | Sigma |
| Glucagon | 1:200 | rabbit polyclonal | Cell Signaling Technology |
| Glial fibrillary acidic protein (GFAP) | 1:500 | rabbit polyclonal | Dako |
| S100 | 1:1000 | rabbit polyclonal | Dako |
| neurofilament 160 kDa (NF-M) | 1:1000 | chick polyclonal | Aves |
| tyrosine hydroxylase (TH) | 1:1000 | rabbit polyclonal | Millipore |
| calcitonin gene-related peptide (CGRP) | 1:2000 | rabbit polyclonal | Calbiochem |
| choline acetyltransferase (ChAT) | 1:30 | rabbit polyclonal | Abcam |
| neuronal protein HuC/HuD | 1:50 | mouse monoclonal | Molecular Probes |
| Pancreatic and duodenal homeobox 1 (PDX1) | 1:400 | rabbit polyclonal | Trans Genic |
| SynCAM | 1:500 | chick polyclonal | MBL |
| Alexa Fluor 488 | 1:1000 | goat anti-rabbit, goat anti-chick, goat anti-guinea pig | Molecular Probes |
| Alexa Fluor 555 | 1:1000 | goat anti-rabbit, streptoavidin conjugated | Molecular Probes |
| Alexa Fluor 633 | 1:1000 | goat anti-rabbit, goat anti-guinea pig, streptoavidin conjugated | Molecular Probes |
| DyLight549 | 1:500 | goat anti-chick | Jackson ImmunoResearch |
Abcam: Cambridge, UK; Aves: Oregon, USA; Calbiochem: Darmstadt, Germany; Cell Signaling Technology: Danvers, USA; Dako: Copenhaven, Denmark; Jackson ImmunoResearch: West Grove, PA, USA MBL: Nagoya, Japan; Millipore: Billerica, USA; Molecular Probes: Merelbeke, Belgium; Sigma-Aldrich; St. Louis, MO, USA; Trans genic: Kumamoto, Japan.