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. 2011 Dec 13;45(1):15–23. doi: 10.1267/ahc.11015

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© 2012 The Japan Society of Histochemistry and Cytochemistry

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — RT-PCR (A, B, C) and real time-PCR (D) analyses of Tfg mRNAs and proteins in the rat retina. A: RT-PCR experiment using a primer set for the common region of cTfg and rTfg. A strong band of 170 bp (arrowhead) and a faint band of 211 bp (arrow) correspond well to the sizes of cTfg and rTfg, respectively. B: RT-PCR experiment using a primer set specific for rTfg. A single band of 426 bp is seen. C: RT-PCR experiment using a primer set specific for β-actin. β-actin mRNA was evenly expressed in all tissues examined. D: Real-time PCR demonstrated high expression levels of rTfg mRNA in the brain, and this was normalized to 100%. M indicates a marker. 1; brain, 2; heart, 3; lung, 4; colon, 5; liver, 6; retina.