Fig. 6.

Immunohistochemical staining using the purified antibody against the common region of cTFG and rTFG proteins. A: Hematoxylin and eosin staining shows the layers of the rat retina. RCL, layer of rod and cone cells; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. B: Immunohistochemistry shows intense TFG staining in the RCL and OPL and weak staining in the ONL. C: Immunofluorescent histochemistry shows TFG-immunopositivity in the RCL, ONL and OPL. D: Merged photographs of the immunofluorescent histochemistry and bright field photographs. E: Staining is abolished following preincubation of the antibody with the TFG peptide (100 µg/ml). Bar=50 µm.