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. 2012 Apr 2;7(4):e33137. doi: 10.1371/journal.pone.0033137

Figure 2. Nyctalopin is membrane bound in yeast.

Figure 2

A. Schematic diagram of constructs used to determine the localization of nyctalopin in yeast. Nyc-Cub (109.4 kDa) is a fusion protein with the yeast invertase signal sequence (SUC) (20aa), EYFP (217aa), nyctalopin (453aa) and the Cub-LexAVP16 artificial transcription factor (350aa). Alg5-Cub (77.5 kDa) is a yeast ER membrane bound protein fused to Cub-LexAVP16 and is used as a positive control. EYFP-Cub has EYFP fused to Cub-LexAVP16. B. (Left) Confocal images of yeast strain BY4741 expressing either EYFP-Cub or Nyc-Cub showing that EYFP-Cub is in the cytoplasm and Nyc-Cub is localized to the plasma membrane. B. (Right) Western blot of membrane (M) and cytosolic (C) fractions from yeast strain NMY32 transfected with control plasmid (Alg5-Cub), untransfected cells or Nyc-Cub. These data indicate nyctalopin is membrane bound.