Table 3.
Yeast strains used.
| strain | genotype | Protein lacking | source |
|---|---|---|---|
| BY4741 | Matα his3Δ1 leu2Δ0 lys2Δ0 ura 3Δ0 | none | EUROSCARF |
| snm1Δ | Like BY4741 except snm1Δ::KanMx4 | 5′-3′ exonuclease activity | EUROSCARF |
| rad10Δ | Like BY4741 except rad10Δ::KanMx4 | Single-stranded DNA endonuclease (with Rad1p) | EUROSCARF |
| rad1Δ | Like BY4741 except rad1Δ::KanMx4 | Single-stranded DNA endonuclease (with Rad10p) | EUROSCARF |
| rad2Δ | Like BY4741 except rad2Δ::KanMx4 | Single-stranded DNA endonuclease | EUROSCARF |
| xrs2Δ | Like BY4741 except xrs2Δ::KanMx4 | Protein required for DNA repair; Mre11 complex component | EUROSCARF |
| rad5Δ | Like BY4741 except rad5Δ::KanMx4 | DNA helicase proposed to promote replication fork regression during postreplication repair | EUROSCARF |
| rad52Δ | Like BY4741 except rad52Δ::KanMx4 | Protein that stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA | EUROSCARF |
| rad53Δ | Like BY4741 except rad53Δ::KanMx4 | Protein kinase, required for cell-cycle arrest in response to DNA damage | EUROSCARF |
| rev1Δ | Like BY4741 except rev1Δ::KanMx4 | Deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS) | EUROSCARF |
| rev7Δ | Like BY4741 except rev7Δ::KanMx4 | Accessory subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair | EUROSCARF |
| erg3Δ | Like BY4741 except erg3Δ::KanMx4 | C-5 sterol desaturase, catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis | EUROSCARF |
| aus1Δ | Like BY4741 except aus1Δ::KanMx4 | Plasma membrane sterol transporter of the ATP-binding cassette family | EUROSCARF |
| yor1Δ | Like BY4741 except yor1Δ::KanMx4 | Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter mediates export of many different organic anions | EUROSCARF |
| SOD+ | Matα his3Δ1 leu2Δ0 trp1-289 ura3-52 | None | E. B. Gralla, Los Angeles |
| sod2Δ | Like SOD + except sod2::TRP1 | Mn superoxide dismutase | E. B. Gralla, Los Angeles |
| ctt1Δ | Like SOD + except ctt1Δ::TRP1 | cytosolic catalase T | E. B. Gralla, Los Angeles |
| cta1Δ | Like SOD + except cta1Δ::TRP1 | catalase A present in peroxissomal matrix | E. B. Gralla, Los Angeles |