Table 2.
Microsatellite primers developed in this study and their length, GC content, molecular weight, motifs, optimum annealing temperature, size range of fragments and mean number of alleles amplified from the 16 fungal species amplified.
| Nucleotides repeats | Primers | Motif | Length | GC Content (%) | MW | Optimum AT (°C) | Range of fragment size (bp) |
|---|---|---|---|---|---|---|---|
| Di | (CT)7G | CT | 15 | 53.3 | 4420.9 | 40 | 400–2000 |
| (CA)7T | CA | 15 | 46.7 | 4458.9 | 52 | 300–1700 | |
| (AG)7C | AG | 15 | 53.3 | 4724.1 | 48 | 200–2500 | |
| Tri | (CTG)5 | CTG | 15 | 66.7 | 4550.9 | 52 | 450–2000 |
| (AGG)5 | AGG | 15 | 66.7 | 4796.1 | 52 | 350–2000 | |
| (TCC)5 | TCC | 15 | 66.7 | 4350.8 | 50 | 400–2000 | |
| (ACG)5 | ACG | 15 | 66.7 | 4596.0 | 50 | 400–2000 | |
| (TGG)5 | TGG | 15 | 66.7 | 4751.1 | 50 | 350–1500 | |
| (GTA)5 | GTA | 15 | 33.3 | 4671.1 | 40 | 600–2000 | |
| (ATG)5 | ATG | 15 | 33.3 | 4671.1 | 50 | 200–3000 | |
| (TAC)5 | TAC | 15 | 33.3 | 4470.9 | 40 | 700–1500 | |
| (TGC)5 | TGC | 15 | 66.7 | 4550.9 | 40 | 1000–1800 | |
| (GCT)5 | GCT | 15 | 66.7 | 4550.9 | 52 | 600–2000 | |
| (TGT)5 | TGT | 15 | 33.3 | 4626.0 | 40 | 400–1800 | |
| Tetra | (TGAC)4 | TGAC | 16 | 50.0 | 4881.2 | 55 | 400–2500 |
| (GTCA)4 | GTCA | 16 | 50.0 | 4881.2 | 50 | 400–1800 | |
| (TAGG)4 | TAGG | 16 | 50.0 | 5041.3 | 50 | 200–2000 | |
| (TTTC)4 | TTTC | 16 | 25.0 | 4745.1 | 40 | 300–2000 | |
| (TACC)4 | TACC | 16 | 50.0 | 4721.1 | 45 | 550–2000 | |
| (GGTT)4 | GGTT | 16 | 50.0 | 5005.3 | 40 | 550–2500 | |