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Aβ aggregation monitored via dot blot. A 50 μM Aβ1-40 sample was incubated in PBS (pH 7.4) at 37 °C in the presence (+) or absence (−) 3 x Brilliant Blue G (BBG) inhibitor. Samples were taken on the indicated days and spotted on a nitrocellulose membrane. Oligomer-specific A11 antibody and Aβ-sequence specific antibodies 4G8 and 6E10 were used to detect aggregates. Reprinted with permission from [57]. Copyright (2011) American Chemical Society.
