Figure 1.

Effects of peptides on Circular dichroism (CD) spectra of gelatin in the wavelength region from 250–190 nm. Gelatin solution was treated with peptides at an increasing molar ratio of gelatin to peptide from 11:1 to 11:3 and incubated for 10 min at 25 °C. (A) (KW)₄; (B) HPA3NT3-analog; (C) NRC-16; (D) Magainin-II; and (E) Reduced glutathione (GSH). CD spectra of 0.1% gelatin in the absence of peptides (solid line). Peptide concentrations were as follows: 50 μM (dotted line), 100 μM (dashed line), and 200 μM (dashed-dotted line). (F) CD spectra of gelatin in the absence (solid line) or presence of 1 mM SDS (dotted line), 5 mM SDS (dashed line), and 10 mM SDS (dashed dotted line).