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. 2012 Mar 8;13(3):3245–3276. doi: 10.3390/ijms13033245

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© 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Multiplex Ligation-dependent Probe Amplification (MLPA) analysis of the Duchenne Muscular Dystrophy (DMD) gene. Abscissa represents DMD gene and control probes (c); ordinate represents fluorescent intensity of amplification. For each probe, the ratio <0.75 stands for deletion; and the ratio >1.3 stands for duplication. (a) MLPA analysis showing a heterozygous deletion of exons 46–50 (ratio < 0.75) of the DMD gene in the mother of an affected patient; (b) Normal control (075 < ratio < 1.3).