Table 2.

Inhibition parameters of several putative CBIs on bean callus growth. I₁₀, I₅₀ and I₉₀ values were calculated as the concentration of inhibitor able to inhibit the increase in dry weigth (DWt) by 10%, 50% and 90% respectively. The active concentration range was expressed as the ratio I₉₀/I₁₀. Dry weigth (DWt)/fresh weight (FWt) ratio was estimated at I₅₀ and I₉₀ concentrations. The DWt/FWt from callus cultures growing in the absence of any inhibitor was 0.046. Cellulose content of cell walls isolated from calluses was measured after 30 days culture in the presence of the inhibitors at I₅₀ concentration. Cellulose content in control was 212.6 ± 23.6 μg mg⁻¹ CW. Values are means ± SD of 9 measurements.

	Inhibition Parameters				DWt/FWt
CBI	I10	I50	I90	Active Concentration Range (I90/I10)	DWt/FWt (I50)	DWt/FWt (I90)	Cellulose (μg mg−1 CW)
CGA 325′615	<0.1 nM	0.5 nM	10 nM	100	0.048	0.052	241.7 ± 33.3
AE F150944	0.8 nM	1 nM	>20 mM	~25000	0.053	~0.064	277.2 ± 18.7 *
Flupoxam	0.2 nM	2 nM	400 nM	2000	0.048	0.058	216.3 ± 5.5
Triazofenamide	4 nM	15 nM	100 nM	25	0.047	0.046	258.5 ± 29.3 *
Oxaziclomefone	0.6 nM	30 nM	>1 μM	~1667	0.048	~0.052	229 ± 22.7
Dichlobenil	0.2 μM	0.5 μM	1 μM	5	0.032	0.048	220.4 ± 18.4
Quinclorac	4 μM	10 μM	20 μM	5	0.050	0.053	247.9 ± 4.1
Compound 1	20 nM	20 μM	200 μM	10000	0.055	0.060	328.5 ± 37.2 *

^*Values statistically different of control by Tukey test p < 0.05.