Figure 3.

Surface plasmon resonance binding analysis of the α5β1 headpiece fragment to Fn7–10. Purified and unclasped α5β1 headpiece fragment at a concentration of 100 nM was flowed for 60 s over the surface of immobilized Fn7–10 (∼1,400 resonance units [RU]) in a buffer containing 1 mM MnCl₂. The integrin was preincubated with SG/19 Fab (10-fold molar excess; dotted line) or mAb 16 IgG (fourfold molar excess; gray line) or left untreated (solid line). (inset) Concentration-dependent binding sensorgrams of the same unclasped α5β1 headpiece fragment onto Fn7–10 in a buffer containing 1 mM CaCl₂ and 1 mM MgCl₂. This experiment was completed once.