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. 2001 Apr 10;98(9):5146–5151. doi: 10.1073/pnas.081080298

Figure 1.

Figure 1

Secretion of IL-15 by recombinant vaccinia viruses. Recombinant vaccinia viruses with the coding region of human IL-15 gene under the control of vaccinia virus P7.5 early–late promoter were created as described in Materials and Methods. In IL-15(IS) vaccinia virus, the signal sequence of murine IgG κ light chain mediates the secretion of IL-15, whereas in IL-15(NS) vaccinia virus, the secretion is mediated by the native signal sequence. CV-1 cells were infected with the recombinant viruses at a multiplicity of infection of 0.001, and the secreted IL-15 in culture supernatants was measured by ELISA.