Figure 4.

Cytokine expression in tissues of infected mice. Nude mice were inoculated i.v. with 10⁶ pfu of the respective recombinant vaccinia viruses and tissues were harvested 36 h after infection. Total cellular RNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4), and the cytokine mRNA levels were determined by RPA as described in Materials and Methods. Each sample represents tissue RNA pooled from three animals. The expression levels of ribosomal gene L32 served as an internal control for RNA integrity. Data are representative of two similar experiments performed.