Figure 6.
Effects of recombinant vaccinia virus infection on chemokine expression in tissues of infected mice. Nude mice were inoculated i.v. with 106 pfu of the respective recombinant vaccinia viruses and tissues were harvested 36 h (A) and 10 days (B) after infection. Total cellular RNA was then isolated from lung (lanes 1), spleen (lanes 2), brain (lanes 3), and liver (lanes 4), and the chemokine mRNA levels were determined by RPA as described in Materials and Methods. Each sample represents tissue RNA pooled from three animals. The expression levels of ribosomal gene L32 serve as internal controls for RNA integrity. Data are representative of two similar experiments performed.