Table 1.

Production of isoflavonoids in the suspension culture of Trifolium pratense.

Elicitor concentration (μmol L−1)	Time after elicitation (hours)	Genistin (mg g−1 DW)	Daidzein (mg g−1 DW)	Genistein (mg g−1 DW)	Formononetin (mg g−1 DW)	Biochanin A (mg g−1 DW)
0 (control)	6	4.61 ± 0.03	3.32 ± 0.08	0.31 ± 0.05	3.69 ± 0.09	0.28 ± 0.04
	24	4.61 ± 0.03	3.32 ± 0.08	0.31 ± 0.05	3.69 ± 0.09	0.28 ± 0.04
	48	4.61 ± 0.03	3.32 ± 0.08	0.31 ± 0.05	3.69 ± 0.09	0.28 ± 0.04
	168	6.30 ± 0.05	3.41 ± 0.06	1.19 ± 0.02	3.91 ± 0.06	0.31 ± 0.02
1	6	7.21* ± 0.09	2.61* ± 0.10	0.52 ± 0.10	4.78* ± 0.10	0.29 ± 0.08
	24	8.89* ± 0.04	5.99* ± 0.09	0.88* ± 0.05	1.12* ± 0.08	0.12 ± 0.12
	48	11.60* ± 0.07	8.31* ± 0.04	1.50* ± 0.06	5.83* ± 0.05	0.21 ± 0.07
	168	1.31* ± 0.12	0.32* ± 0.07	0.23* ± 0.04	0.71* ± 0.04	0.09* ± 0.03
10	6	11.49* ± 0.08	2.98* ± 0.04	1.32* ± 0.08	7.31* ± 0.05	0.28 ± 0.09
	24	7.71* ± 0.10	3.13 ± 0.09	0.31 ± 0.02	6.53* ± 0.09	0.00* ± 0.00
	48	7.39* ± 0.05	2.49* ± 0.06	0.48 ± 0.13	3.62 ± 0.11	0.11* ± 0.02
	168	2.20* ± 0.07	0.62* ± 0.10	0.19* ± 0.05	0.42* ± 0.05	0.11* ± 0.05
100	6	5.11* ± 0.04	2.67* ± 0.15	0.42 ± 0.06	0.59* ± 0.07	0.29 ± 0.07
	24	7.19* ± 0.09	2.92* ± 0.08	0.00* ± 0.00	0.72* ± 0.04	0.19 ± 0.04
	48	8.78* ± 0.11	2.42* ± 0.03	0.14* ± 0.05	2.90* ± 0.06	0.11* ± 0.02
	168	0.67* ± 0.07	0.23* ± 0.05	0.32* ± 0.04	1.89* ± 0.05	0.08* ± 0.12

Levels marked with * are statistically significantly different compared with the control each value represents the mean of three samples ± standard deviation.