Table 1.

Kinetic characterization of recombinant Pye843

Substrates	Km (μM)	kcat (S-1)	Enzyme activity (U/mg)
ρ-nitrophenyl acetate	14.1 ± 3.2	289.1 ± 3.0	772.9 ± 3.4
ρ-nitrophenyl butyrate	31.6 ± 4.0	163.3 ± 6.2	408.3 ± 9.2
ρ-nitrophenyl caprylate	33.2 ± 2.1	149.7 ± 8.3	388.6 ± 5.4
ρ-nitrophenyl caproate	57.6 ± 1.9	88.9 ± 3.5	115.7 ± 4.3
ρ-nitrophenyl decanoate	99.7 ± 9.3	69.1 ± 2.2	56.9 ± 3.2
ρ-nitrophenyl laurate	130.9 ± 7.2	49.1 ± 6.6	34.5 ± 2.1