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. 2012 Jan 24;9:30. doi: 10.1186/1743-422X-9-30

Figure 1.

Figure 1

Permissiveness of cell lines for HCVpp and HCVcc infection. (A) Indicated cell lines were infected with equal amounts of JFHpp, H77pp or VSVGpp. HCVpp entry (relative light units (RLU) ± SD for triplicate samples determined 72 h p.i.) relative to Huh7-1 cells was calculated by subtracting cell line specific background and mock-control signals and then normalizing for cell line-specific VSVGpp entry levels. Results are graphed as a percentage of entry achieved in Huh7-1 infected cultures. (B) Indicated cell lines were infected with HCVcc at an MOI of 2.0 FFU/cell. Intracellular RNA was collected 72 h p.i. and HCV RNA was quantified by RTqPCR, normalized to GAPDH and is displayed as HCV RNA copies/μg total cellular RNA (means ± SD for triplicate samples). Significant increases in HCV RNA levels compared to CHO cells (one-way ANOVA and Tukey's post hoc t test) are denoted as * p value < 0.001. Dashed line represents background levels of non-specific bound HCV RNA.