Table 2. Comparison of IS2404 PCR with conventional LAMP for the detection of Mycobacterium ulcerans using different DNA templates.
Conventional LAMP | |||||||||
Unboiled extract | Boiled extract | Purified extract | |||||||
(+) | (−) | Total | (+) | (−) | Total | (+) | (−) | Total | |
IS2404 PCR (+) | 12 | 9 | 21 | 9 | 12 | 21 | 21 | 0 | 21 |
IS2404 PCR (−) | 0 | 9 | 9 | 0 | 9 | 9 | 0 | 9 | 9 |
Total | 12 | 18 | 30 | 9 | 21 | 30 | 21 | 9 | 30 |
Positivity | 40% | 30% | 70% | ||||||
*Sensitivity | 100% | 100% | 100% |
*: Sensitivity as compared with IS2404 PCR
LAMP (Loop mediated isothermal amplification).