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. 2012 Apr 3;7(4):e33031. doi: 10.1371/journal.pone.0033031

Figure 3. Effect of SC and CSP on DNA fragmentation.

Figure 3

The T47D cells were treated with SC (10 µg/ml), CSP (10 µg/ml) or Dauno (100 µM) for 24 h, thereafter DNA fragmentation was analyzed by comet assay (A), agarose gel electrophoresis (B) and diphenylamine assay (C). Basal DNA damage from untreated cells, DNA damage from SC or CSP cells (A). Data are from a single experiment and representative of 50 randomly selected cells stained with ethidium bromide (EtBr). Data illustrated in (B) are from a single experiment and representative of three separate experiments, while data in (C) are expressed as mean ± S.E.M. of three separate experiments. ***p<0.001 vs. control (untreated cells).