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. 2012 Apr 3;7(4):e33031. doi: 10.1371/journal.pone.0033031

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De Stefano et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Detection of dead and dying cells by FACS (A). Cells treated overnight with SC, CSP and cisPt were stained with ΔΨ_m-sensitive dye DiOC₆(3) and the vital dye propidium iodide (PI). The white portions of the columns refer to the DiOC₆(3)^low/PI⁺ population (dead) and the remaining part of the column corresponds to the DiOC₆(3)^low/PI^- (dying) population. Results are means ± S.E.M. of three independent experiments. Expression profile of apoptosis-related proteins (B). Cell lysates from HeLa cells, treated with SC (10 µM) and CSP (10 µM) overnight, were analyzed by human apoptosis profiler. Data are expressed as means ± S.D. of two independent experiments.