Table 3. Specific [3H]ketanserin, [3H]muscimol, [3H]CP 55,940, [3H]WAY 100.635 and [3H]MK-801 binding in different brain regions.
| Region | WT | Nrg1 TM HET | ||||||
| Vehicle | CBD 1 | CBD 50 | CBD 100 | Vehicle | CBD 1 | CBD 50 | CBD 100 | |
| [3H]ketanserin (5-HT2A receptors) | ||||||||
| Prelimbic cortex | 30.4±2.4 | 30.9±0.3 | 30.3±1.9 | 31.3±1.4 | 31.4±0.9 | 30.1±0.8 | 31.3±1.1 | 31.0±1.0 |
| Cingulate cortex | 30.6±0.9 | 30.6±0.7 | 30.5±1.2 | 30.2±0.8 | 30.7±1.0 | 31.4±0.5 | 30.0±0.5 | 30.1±0.3 |
| Caudate putamen | 28.7±0.8 | 28.4±1.4 | 29.0±2.5 | 27.9±0.8 | 28.1±1.0 | 29.3±1.0 | 27.8±0.8 | 27.8±0.8 |
| Hippocampus | 25.8±0.5 | 25.4±0.5 | 25.5±0.8 | 25.3±0.7 | 25.3±1.1 | 26.1±0.3 | 25.2±1.0 | 25.0±0.9 |
| Substantia nigra | 34.6±0.9 | 33.4±1.8 | 31.3±1.0# | 32.0±2.6 | 32.3±1.1* | 34.1±0.9 | 33.0±2.0 | 32.4±0.7 |
| [3H]muscimol (GABAA receptors) | ||||||||
| Prelimbic cortex | 21.8±0.1 | 21.7±0.8 | 21.9±1.3 | 21.5±1.5 | 20.8±0.9 | 21.3±1.1 | 22.2±1.1 | 21.1±1.9 |
| Cingulate cortex | 22.7±0.3 | 23.0±1.3 | 23.3±1.4 | 22.4±0.9 | 22.3±1.0 | 22.7±0.6 | 23.3±1.3 | 22.3±2.1 |
| Caudate putamen | 20.1±0.3 | 20.2±0.7 | 20.2±0.8 | 19.8±0.6 | 19.7±0.6 | 20.0±0.4 | 20.4±0.6 | 19.7±1.2 |
| Dorsolateral septum | 19.7±0.8 | 19.5±0.7 | 19.4±0.9 | 19.7±1.0 | 19.3±0.5 | 19.0±0.8 | 19.2±0.7 | 19.4±1.2 |
| Retrosplenial granular cortex | 20.5±0.8 | 20.5±0.6 | 20.2±0.7 | 20.5±0.8 | 19.9±0.3 | 20.4±0.7 | 21.3±0.6## | 20.3±0.5 |
| CA1 | 22.3±0.9 | 22.2±1.2 | 21.6±1.5 | 22.0±1.2 | 21.6±1.0 | 21.6±1.0 | 22.4±1.2 | 21.9±1.2 |
| Dentate gyrus | 22.3±0.9 | 22.4±1.1 | 22.0±1.5 | 22.5±1.3 | 22.1±1.5 | 22.1±0.7 | 23.0±1.4 | 22.6±1.3 |
| Thalamus | 23.4±1.1 | 23.4±0.9 | 23.4±1.6 | 23.2±1.1 | 22.7±1.1 | 22.8±0.7 | 24.1±1.5 | 23.0±1.1 |
| [3H]CP 55,940 (CB1 receptors) | ||||||||
| Cingulate cortex | 27.0±0.6 | 26.4±0.5 | 26.8±1.0 | 26.6±0.6 | 26.4±0.4 | 26.8±0.4 | 27.2±1.0 | 27.0±0.7 |
| Caudate putamen | 26.3±0.3 | 26.4±0.7 | 26.6±0.6 | 26.1±0.8 | 26.3±0.4 | 26.8±0.4 | 26.6±0.3 | 26.6±0.3 |
| Dorsolateral septum | 25.5±0.9 | 25.3±1.1 | 25.3±1.1 | 25.1±1.5 | 25.4±0.5 | 25.4±1.2 | 25.7±0.9 | 25.8±0.6 |
| Ventrolateral septum | 25.4±1.1 | 25.4±1.4 | 26.6±1.3 | 25.7±1.1 | 26.4±1.0 | 25.5±1.5 | 25.8±2.1 | 25.2±1.1 |
| Hippocampus | 26.3±0.5 | 26.0±0.6 | 26.1±0.5 | 25.9±1.2 | 26.2±0.9 | 26.2±0.6 | 26.7±1.0 | 26.6±0.7 |
| Substantia nigra | 105.6±9.7 | 101.0±5.2 | 96.4±8.9 | 97.9±9.6 | 106.0±5.7 | 102.5±9.2 | 102.6±3.2 | 107.6±12.7 |
| [3H]WAY 100,635 (5-HT1A receptors) | ||||||||
| Cingulate cortex | 25.9±1.8 | 25.5±1.0 | 26.1±0.7 | 26.3±1.0 | 26.1±0.8 | 25.8±0.9 | 25.6±1.2 | 25.5±0.7 |
| Hippocampus | 85.3±9.7 | 86.5±5.7 | 85.2±6.7 | 84.5±10.6 | 86.7±2.8 | 88.4±5.2 | 86.3±4.7 | 83.1±4.6 |
| Lateral septum | 31.3±2.1 | 32.1±1.7 | 32.3±0.8 | 31.3±2.3 | 32.3±1.4 | 31.9±1.1 | 31.4±1.4 | 31.6±0.6 |
| Retrosplenial granular cortex | 30.1±3.1 | 28.6±1.5 | 28.8±1.5 | 29.8±2.4 | 31.1±2.4 | 30.0±0.7 | 30.5±1.8 | 29.3±2.0 |
| Nucleus of the vertical limb of the diagonal band | 32.3±3.0 | 33.2±2.0 | 33.3±1.1 | 31.4±2.7 | 32.0±2.6 | 34.1±1.3 | 33.6±1.8 | 32.8±2.3 |
| [3H]MK-801 (NMDA receptors) | ||||||||
| Cingulate cortex | 36.9±0.9 | 37.8±1.6 | 38.1±1.7 | 37.6±0.7 | 37.5±0.5 | 38.6±0.7 | 38.3±1.0 | 38.1±0.8 |
| Caudate putamen | 31.4±1.0 | 32.1±1.1 | 32.1±1.3 | 32.4±0.8 | 32.2±0.4 | 32.2±0.5 | 32.5±1.0 | 31.8±1.2 |
| Hippocampus | 109.9±2.3 | 111.6±4.6 | 111.9±5.1 | 111.2±2.5 | 110.4±2.3 | 113.0±3.9 | 114.8±4.9 | 111.7±3.6 |
| Dorsolateral septum | 34.0±2.2 | 33.9±1.3 | 35.5±2.0 | 34.0±2.1 | 35.5±0.4 | 36.0±1.1 | 33.6±2.5 | 33.2±1.1 |
| Retrosplenial granular cortex | 32.4±1.4 | 33.2±1.5 | 33.6±1.6 | 32.6±1.0 | 32.8±0.4 | 33.2±1.0 | 34.5±1.1 | 33.5±1.0 |
[3H]ketanserin, [3H]muscimol, [3H]CP 55,940, [3H]WAY 100,635 and [3H]MK-801 binding 48 h after the last of 21 daily injections of CBD (1, 50 or 100 mg/kg) (on WH day). Data represent mean binding density nCi/mg tissue (± S.E.M.). Significant one-way ANOVA (split by corresponding factor) results are shown:
*
P<0.05 (vs. WT receiving corresponding treatment),
#
P<0.05,
##
P<0.01 (vs. vehicle of corresponding genotype).