Figure 8. Activation of Caspase-9/caspase-3, and DNA damage.

Cleavage of procaspase into cleaved low molecular weight protein as activation of caspases, was observed in cytosolic fraction and OGG formation, marker for oxidative DNA damage were assessed by immunoblotting (A). β-Actin was used as internal loading control and the densitometric values in bar graph (B) are normalized with it. Densitometry of bands was done using ImageJ software (V1.41o, NIH, USA). Values are represented as compared to vehicle control in fold change. Level of significance is denoted as */# P<0.05, **/## P<0.01 and ***/### P<0.001. *, compared to vehicle control and #, compared to nimesulide stress.