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. 2012 Apr 3;7(4):e34355. doi: 10.1371/journal.pone.0034355

Figure 3. EMSA experiments for the assessment of AP-4 / C type / T type probe binding ability on addition of unlabelled AP-4 consensus oligos as competitor.

Figure 3

Panel (A): Shows the EMSA experiments carried out to assess the displacement of the probe complexed with the protein in the presence of excess molar concentrations (10, 50 & 100×) of the unlabelled AP-4 oligonucleotide as indicated. The probe-protein complex was generated using the labeled AP-4 consensus (Lanes 1–5), C (Lanes 6–10) and T probes (Lanes 11–15). Panel (B): The bar diagram shows the densitometric profile of the autoradiograph depicted in Figure 2, Panel c. The labeled AP-4 consensus (Lanes 1–5), C probe (Lanes 6–10) and T probe (Lanes 11–15) were incubated with the nuclear lysate. The bars represent the mean ± SD of three experiments.